Fenofibrate treatment changed polyacrylamide gel electrophoresis (PAGE) patterns of dyslipidemias and their components
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منابع مشابه
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives.
The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms. While advances in mass spectrometry have been critical, developments made in two-dimensional PAGE (2D-PAGE) have also played a major role in enabling proteomic...
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Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...
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MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...
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Twenty-three control samples of CSF were analyzed by vertical column electrophoresis on acrylamide gel. Quantitation results using a microdensitometer are presented. The normal protein pattern shows 15 constant bands which coincide in mobility and in many cases in staining pattern with serum proteins. The haptoglobin peaks, except for the band of the 1-1 type and the leading band of the 2-1 typ...
متن کاملFlat gel polyacrylamide electrophoresis of porcine mycoplasmas.
The flat gel acrylamide electrophoresis technique was standardized and applied to the comparison of four species of porcine mycoplasmas. Clear differences were observed between these species, and differences were seen among the strains of Mycoplasma hyosynoviae. The clarity of the patterns and the number of bands developed was influenced by the amount of protein in the extract and the age of th...
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ژورنال
عنوان ژورنال: The Journal of Japan Atherosclerosis Society
سال: 2002
ISSN: 0386-2682,2185-8284
DOI: 10.5551/jat1973.29.3_33